Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: Differentially expressed circRNAs in the plasma of ESCC patients.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Clinical Proteomics

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: Differentially expressed circRNAs in ESCC patients. (A) The scatter plot of differentially expressed circRNAs. Green lines represent fold change of 2.0. (B) Volcano plot of differentially expressed circRNAs. The green vertical lines correspond to a fold change of 2.0, and the green horizontal line corresponds to a P -value of 0.05. (C) Hierarchical clustering of differentially expressed circRNAs. Each column represents a sample and each row represents a circRNA. The color reflects the expression level of circRNAs and changes from red (high) to black (medium) to green (low). (D) Bar plot shows the distributions of the differentially expressed circRNAs in human chromosomes.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: CircRNA–miRNA–mRNA interaction network in ESCC. CircRNAs are represented by diamonds; miRNAs are shown as triangles; the DEGs are represented by ellipses. Red represents upregulated expression, and blue color represents downregulated expression. circRNA, circular RNA; miRNA, microRNA; ESCC, esophageal squamous cell carcinoma; DEGs, differentially expressed genes.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: Sub-network of circRNAs, miRNAs, and hub genes. circRNAs are represented by diamonds; miRNAs are shown as triangles; hub genes are represented by ellipses. Red represents upregulated expression, and blue color represents downregulated expression. circRNA, circular RNA; miRNA, microRNA.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Primer details for quantitative real-time reverse transcription–polymerase chain reaction of circRNA_000585.

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Heatmap of circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens (P1–3) and matched para-cancer specimens (C1–3) from three patients with CCA. Each block represents different circRNA expression levels (red represents high expression; green represents low expression).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: RNA Expression, Blocking Assay, Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Fold change in circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens and matched para-cancer specimens from three patients with CCA. (A) scatter plot (dots above the upper line represent fold-change >1.5, dots below the lower line represent fold-change >–1.5); and (B) volcano plot of circRNA expression (red dots represent fold-change >1.5 or >–1.5).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: RNA Expression, Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Vertical scatter plot showing elevated circRNA_000585 expression in cholangiocarcinoma (CCA) tumour tissue versus matched para-cancer tissue from 15 patients with CCA (central horizontal line represents mean, upper and lower horizonal lines represent SD; P = 0.003 between groups).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Association between circRNA_000585 expression and clinicopathological characteristics in 15 patients with cholangiocarcinoma.

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Expressing

Journal: Biological Research

Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

doi: 10.1186/s40659-020-00299-y

Figure Lengend Snippet: The list of differential expressed circRNAs in plasma exosome samples from patients with GD and healthy control subjects

Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

Techniques: Clinical Proteomics, Control

Journal: Biological Research

Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

doi: 10.1186/s40659-020-00299-y

Figure Lengend Snippet: The circRNA-miRNA-mRNA regulatory network of hsa_circRNA_000102. CircRNA, miRNA, and mRNA are indicated as spheres in brown, red, blue color, respectively. CircRNA circular RNA, miRNA microRNA, mRNA messenger RNA

Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

Techniques:

Journal: Biological Research

Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

doi: 10.1186/s40659-020-00299-y

Figure Lengend Snippet: The top 10 significantly enriched terms of biological process by GO analysis of hsa_circRNA_000102 associated genes. GO analysis was divided into three parts: biological process, cell component and molecular function

Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

Techniques:

Journal: Biological Research

Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

doi: 10.1186/s40659-020-00299-y

Figure Lengend Snippet: The top 10 significant enriched pathways by KEGG pathway analysis of hsa_circRNA_000102 associated genes

Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

Techniques:

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

doi: 10.3389/fnmol.2019.00196

Figure Lengend Snippet: The primers used in qRT-PCR experiments.

Article Snippet: Hybridization solution (50 μl) was dispensed into the gasket slide (Agilent, Cat No. G2534-60003, United States) and assembled to a mouse circRNA microarray (8 × 15K, Arraystar) slide.

Techniques:

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

doi: 10.3389/fnmol.2019.00196

Figure Lengend Snippet: Top 10 up- and down-regulated DEcircRNAs in the hippocampus of Nrf2 (−/−) mice.

Article Snippet: Hybridization solution (50 μl) was dispensed into the gasket slide (Agilent, Cat No. G2534-60003, United States) and assembled to a mouse circRNA microarray (8 × 15K, Arraystar) slide.

Techniques:

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

doi: 10.3389/fnmol.2019.00196

Figure Lengend Snippet: QRT-PCR validation of the expression levels of candidate circRNAs (A) and lncRNAs (B) . * p < 0.05 and ∗∗ p < 0.01. The deep red column indicates the expression status of lncRNAs through microarray analyses; the blue column indicates the expression status of lncRNAs through qRT-PCR experiments. n = 3.

Article Snippet: Hybridization solution (50 μl) was dispensed into the gasket slide (Agilent, Cat No. G2534-60003, United States) and assembled to a mouse circRNA microarray (8 × 15K, Arraystar) slide.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Microarray

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

doi: 10.3389/fnmol.2019.00196

Figure Lengend Snippet: DEcircRNA-miRNA-DEceRNA interaction subnetworks of up-regulated circRNAs and down-regulated circRNAs in the Nrf2 (–/–) hippocampus. (A) Subnetwork of mmu_circRNA_44531 in the Nrf2 (–/–) hippocampus. (B) Subnetwork of mmu_circRNA_34132 in the Nrf2 (–/–) hippocampus. (C) Subnetwork of mmu_circRNA_000903 in the Nrf2 (–/–) hippocampus. (D) Subnetwork of mmu_circRNA_018676 in the Nrf2 (–/–) hippocampus. (E) Subnetwork of mmu_circRNA_45901 in the Nrf2 (–/–) hippocampus. (F) Subnetwork of mmu_circRNA_33836 in the Nrf2 (–/–) hippocampus. (G) Subnetwork of mmu_circRNA_34137 in the Nrf2 (–/–) hippocampus. (H) Subnetwork of mmu_circRNA_34106 in the Nrf2 (–/–) hippocampus. (I) Subnetwork of mmu_circRNA_008691 in the Nrf2 (–/–) hippocampus. (J) Subnetwork of mmu_circRNA_003237 in the Nrf2 (–/–) hippocampus. Yellow nodes indicate DEcircRNAs. Magenta and green nodes indicate miRNAs sponged by DEcircRNAs and the gene ID of their DEceRNAs, respectively. Edges represent interactions.

Article Snippet: Hybridization solution (50 μl) was dispensed into the gasket slide (Agilent, Cat No. G2534-60003, United States) and assembled to a mouse circRNA microarray (8 × 15K, Arraystar) slide.

Techniques: